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The MTHFD1L encoded enzyme is a mono-functional enzyme with 10-formyl-THF synthetase activity and the MTHFD2L encoded enzyme is a bi-functional enzyme with 5,10-methylene-THF dehydrogenase and 5,10-methenyl-THF cyclohydrolase activities

Given the central role of GPX4 in suppressing lipid peroxidation, these findings further suggest that Prx6 may exert substantial influence on ferroptosis-related pathologies through its regulation of GPX4 expression

Therefore, meaningful in vivo experiments cannot be performed with HCMV in unmodified small animal models